Journal Information
Journal ID (publisher-id): BM
Journal ID (nlm-ta): Biochem Med (Zagreb)
Title: Biochemia Medica
Abbreviated Title: Biochem. Med. (Zagreb)
ISSN (print): 1330-0962
ISSN (electronic): 1846-7482
Publisher: Croatian Society of Medical Biochemistry and Laboratory Medicine
Article Information
Copyright statement: ©Croatian Society of Medical Biochemistry and Laboratory Medicine.
Copyright: 2022, Croatian Society of Medical Biochemistry
License (open-access):
This is an Open Access article distributed under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Date received: 16 February 2022
Date accepted: 06 June 2022
Publication date (electronic): 05 August 2022
Publication date (print): 01 October 2022
Volume: 32
Issue: 3
Electronic Location Identifier: 030802
Publisher ID: bm-32-3-030802
DOI: 10.11613/BM.2022.030802
Utility of serum indices in a particular case of serum protein electrophoresis
Cristina Vicidomini[1]
Antonia De Luisi[1]
Andrea Mega[5]
[1] Clinical Biochemical Laboratory, Hospital of Bolzano, Bolzano, Italy
[2] Northern Ireland Blood Transfusion Service, Belfast, UK
[3] Department of Internal Medicine V, Medical University of Innsbruck, Innsbruck, Austria
[4] Department of Industrial Engineering, University of Trento, Trento, Italy
[5] Division of Gastroenterology, Hospital of Bolzano, Bolzano, Italy
Author notes:
[*] Corresponding author: massimo.daves@sabes.it
Screening and measurement of monoclonal (M) proteins are commonly performed using capillary zone electrophoresis (CZE). The identification of M-protein or monoclonal component (CM) is an essential requirement for diagnosis and monitoring of monoclonal gammopathies. The detection of CM has been largely improved by CZE. Capillary electrophoresis estimates CM more accurately, because absence of variation due to different dye binding affinities of proteins as instead seen with agarose gel electrophoresis. However, interferences can be present in CZE. This occurs because all substances absorbing at 200 nm can be identified. Recognition and handling of specimens exhibiting such interferences is essential to ensure accurate diagnostic and patient safety. We herein report on an unusual case of serum protein electrophoresis, to highlight that laboratory staff must be aware of and familiarise with the information provided by laboratory instruments. For example, in the case of serum indices, about specimen quality.
Keywords: capillary zone electrophoresis; interferences; serum indices